产品内容
本酶催化的反应为(1) (E)-2-Methylbutanal Oxime + [Reduced NADPH---Hemoprotein Reductase] + O2 = 2-Hydroxy-2-Methylbutanenitrile + [Oxidized NADPH---Hemoprotein Reductase] + 2 H2O (Overall Reaction);;(1a) (E)-2-Methylbutanal Oxime = (Z)-2-Methylbutanal Oxime;;(1b) (Z)-2-Methylbutanal Oxime = 2-Methylbutanenitrile + H2O;;(1c) 2-Methylbutanenitrile + [Reduced NADPH---Hemoprotein Reductase] + O2 = 2-Hydroxy-2-Methylbutanenitrile + [Oxidized NADPH---Hemoprotein Reductase] + H2O;;(2) (E)-2-Methylpropanal Oxime + [Reduced NADPH---Hemoprotein Reductase] + O2 = 2-Hydroxy-2-Methylpropanenitrile + [Oxidized NADPH---Hemoprotein Reductase] + 2 H2O (Overall Reaction);;(2a) (E)-2-Methylpropanal Oxime = (Z)-2-Methylpropanal Oxime;;(2b) (Z)-2-Methylpropanal Oxime = 2-Methylpropanenitrile + H2O;;(2c) 2-Methylpropanenitrile + [Reduced NADPH---Hemoprotein Reductase] + O2 = 2-Hydroxy-2-Methylpropanenitrile + [Oxidized NADPH---Hemoprotein Reductase] + H2O,系统名称为(E)-2-Methylbutanal Oxime,[reduced NADPH---Hemoprotein Reductase]:oxygen Oxidoreductase,这种细胞色素 P-450(血红素-硫醇盐)酶参与生氰糖苷百脉根苷(lotaustralin)和亚麻苦苷(linamarin)的生物合成。它催化三种不同的反应活性——底物由 (E) 构型异构化为 (Z) 构型、脱水以及 C-羟基化。