产品内容
本酶催化的反应为(1) (E)-Indol-3-Ylacetaldehyde Oxime + [Reduced NADPH---Hemoprotein Reductase] + Glutathione + O2 = S-[(E)-N-Hydroxy(indol-3-Yl)acetimidoyl]-L-Glutathione + [Oxidized NADPH---Hemoprotein Reductase] + 2 H2O (Overall Reaction);;(1a) (E)-Indol-3-Ylacetaldehyde Oxime + [Reduced NADPH---Hemoprotein Reductase] + O2 = 1-(1H-Indol-3-Yl)-2-Aci-Nitroethane + [Oxidized NADPH---Hemoprotein Reductase] + H2O;;(1b) 1-(1H-Indol-3-Yl)-2-Aci-Nitroethane + Glutathione = S-[(E)-N-Hydroxy(indol-3-Yl)acetimidoyl]-L-Glutathione + H2O (Spontaneous);;(2) (E)-Phenylacetaldehyde Oxime + [Reduced NADPH---Hemoprotein Reductase] + Glutathione + O2 = S-[(Z)-N-Hydroxy(phenyl)acetimidoyl]-L-Glutathione + [Oxidized NADPH---Hemoprotein Reductase] + 2 H2O (Overall Reaction);;(2a) (E)-Phenylacetaldehyde Oxime + [Reduced NADPH---Hemoprotein Reductase] + O2 = 1-Aci-Nitro-2-Phenylethane + [Oxidized NADPH---Hemoprotein Reductase] + H2O;;(2b) 1-Aci-Nitro-2-Phenylethane + Glutathione = S-[(Z)-N-Hydroxy(phenyl)acetimidoyl]-L-Glutathione + H2O (Spontaneous);;系统名称为(E)-Indol-3-Ylacetaldoxime,[reduced NADPH---Hemoprotein Reductase],glutathione:oxygen Oxidoreductase (Oxime-Hydroxylating)，这种细胞色素 P-450（血红素-硫醇盐）酶参与植物中硫代葡萄糖苷的生物合成。该酶催化来源于 L-色氨酸、L-苯丙氨酸和 L-酪氨酸的芳香醛肟的 N-羟基化，形成酸式硝基（aci-nitro）中间体，该中间体与谷胱甘肽非酶促反应生成 N-烷基硫代羟肟酸酯加合物，即硫代葡萄糖苷的关键前体。在缺乏谷胱甘肽时，该酶会发生自杀性失活，可能是由于反应性酸式硝基化合物与活性位点催化残基相互作用所致。
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