产品内容
本酶催化的反应为A Long-Chain Acyl-[Acyl-Carrier Protein] + 2 Reduced Flavodoxin + 3 O2 = Pimeloyl-[Acyl-Carrier Protein] + An N-Alkanal + 2 Oxidized Flavodoxin + 3 H2O (Overall Reaction);;(1a) A Long-Chain Acyl-[Acyl-Carrier Protein] + Reduced Flavodoxin + O2 = A (7S)-7-Hydroxy-Long-Chain-Acyl-[Acyl-Carrier Protein] + Oxidized Flavodoxin + H2O;;(1b) A (7S)-7-Hydroxy-Long-Chain-Acyl-[Acyl-Carrier Protein] + Reduced Flavodoxin + O2 = A (7R,8R)-7,8-Dihydroxy-Long-Chain-Acyl-[Acyl-Carrier Protein] + Oxidized Flavodoxin + H2O;;(1c) A (7R,8R)-7,8-Dihydroxy-Long-Chain-Acyl-[Acyl-Carrier Protein] + Reduced Flavodoxin + O2 = A 7-Oxoheptanoyl-[Acyl-Carrier Protein] + An N-Alkanal + Oxidized Flavodoxin + 2 H2O;;(1d) A 7-Oxoheptanoyl-[Acyl-Carrier Protein] + Oxidized Flavodoxin + H2O = A Pimeloyl-[Acyl-Carrier Protein] + Reduced Flavodoxin + H+，系统名称为Acyl-[Acyl-Carrier Protein],reduced-Flavodoxin:oxygen Oxidoreductase (Pimeloyl-[Acyl-Carrier Protein]-Forming)，一种细胞色素 P-450（血红素-硫醇盐）蛋白。该酶催化各种链长的长链酰基-[酰基载体蛋白]的氧化性 C-C 键断裂，生成庚二酰-[酰基载体蛋白]，这是生物素生物合成的中间体。来自枯草芽孢杆菌（Bacillus subtilis）的该酶的首选底物是棕榈酰-[酰基载体蛋白]，随后生成庚醛作为链烷醛。其机制类似于 EC 1.14.15.6（胆固醇单加氧酶（侧链断裂）），随后是羟基化步骤，该步骤可能是自发进行的 [2]。
![天净沙庚二酰-[酰基载体蛋白]合酶活性测定试剂盒产品图片](https://www.tjs.bio/content/xtheme/tianjingsha/images/noimage.jpg)
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