产品内容
本酶催化的反应为A 14alpha-Methylsteroid + 6 Reduced Ferredoxin [Iron-Sulfur] Cluster + 6 H+ + 3 O2 = A Delta14-Steroid + Formate + 6 Oxidized Ferredoxin [Iron-Sulfur] Cluster + 4 H2O (Overall Reaction);;(1a) A 14alpha-Methylsteroid + 2 Reduced Ferredoxin [Iron-Sulfur] Cluster + 2 H+ + O2 = A 14alpha-Hydroxymethylsteroid + 2 Oxidized Ferredoxin [Iron-Sulfur] Cluster + H2O;;(1b) A 14alpha-Hydroxymethylsteroid + 2 Reduced Ferredoxin [Iron-Sulfur] Cluster + 2 H+ + O2 = A 14alpha-Formylsteroid + 2 Oxidized Ferredoxin [Iron-Sulfur] Cluster + 2 H2O;;(1c) A 14alpha-Formylsteroid + 2 Reduced Ferredoxin [Iron-Sulfur] Cluster + 2 H+ + O2 = A Delta14-Steroid + Formate + 2 Oxidized Ferredoxin [Iron-Sulfur] Cluster + H2O;;系统名称为Sterol,reduced Ferredoxin:oxygen Oxidoreductase (14-Methyl Cleaving)，一种存在于多个细菌物种中的细胞色素 P-450（血红素-硫醇盐）蛋白。该酶参与甾醇生物合成，催化 14α-甲基的羟基化和还原，随后进行第二次羟基化，导致甲酸消除并形成 14(15) 双键。来自荚膜甲基球菌（Methylococcus capsulatus）的该酶通过富含丙氨酸的连接肽与铁氧还蛋白融合。参见 EC 1.14.14.154，甾醇 14α-脱甲基酶。
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