Name: 天净沙胞苷酰-2-羟乙基膦酸甲基转移酶活性测定试剂盒
Brand: 天净沙
SKU: 7-EC2.1.1.308
Availability: InStock

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天净沙胞苷酰-2-羟乙基膦酸甲基转移酶活性测定试剂盒

英文名：Skygen Cytidylyl-2-Hydroxyethylphosphonate Methyltransferase Assay Kit

别名：Fom3; S-Adenosyl-L-Methionine:methylcob(III)alamin:2-Hydroxyethylphosphonate Methyltransferase (Incorrect); 2-Hydroxyethylphosphonate Methyltransferase (Incorrect); S-Adenosyl-L-Methionine:cytidine 5'-{[Hydroxy(2-Hydroxyethyl)phosphonoyl]phosphate} C-Methyltransferase

产品编号：7-EC2.1.1.308

保存温度：-20℃

运输温度：Cold

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货期

目录价

7-EC2.1.1.308

100 T

3-4 Weeks

本酶催化的反应为2 S-Adenosyl-L-Methionine + Cytidine 5'-{[Hydroxy(2-Hydroxyethyl)phosphonoyl]phosphate} + Reduced Acceptor = S-Adenosyl-L-Homocysteine + 5'-Deoxyadenosine + L-Methionine + Cytidine 5'-({Hydroxy[(S)-2-Hydroxypropyl]phosphonoyl}phosphate) + Oxidized Acceptor (Overall Reaction);;(1a) S-Adenosyl-L-Methionine + Cob(I)alamin = S-Adenosyl-L-Homocysteine + Methylcob(III)alamin;;(1b) Methylcob(III)alamin + Cytidine 5'-{[Hydroxy(2-Hydroxyethyl)phosphonoyl]phosphate} + S-Adenosyl-L-Methionine = Cob(III)alamin + Cytidine 5'-({Hydroxy[(S)-2-Hydroxypropyl]phosphonoyl}phosphate) + 5'-Deoxyadenosine + L-Methionine;;(1c) Cob(III)alamin + Reduced Acceptor = Cob(I)alamin + Oxidized Acceptor，系统名称为S-Adenosyl-L-Methionine:cytidine 5'-{[Hydroxy(2-Hydroxyethyl)phosphonoyl]phosphate} C2-Methyltransferase，需要钴胺素。从细菌威德摩尔链霉菌 Streptomyces wedmorensis 中分离的该酶参与磷霉素的生物合成。它是一种自由基S-腺苷-L-甲硫氨酸（SAM）酶，含有一个 [4Fe-4S] 中心和一个甲基钴(III)胺素辅因子。该反应使用两分子SAM：一分子形成5'-脱氧腺苷自由基，另一分子用于甲基化钴胺素辅因子。5'-脱氧腺苷自由基从胞苷5'-{[(2-羟乙基)膦酰]磷酸}的C2位夺取一个氢，形成自由基，后者在SAM和 [4Fe-4S] 簇的另一侧与甲基钴(III)胺素上的甲基反应，经构型翻转生成甲基化产物的(S)-异构体和钴(III)胺素。[4Fe-4S] 簇和钴(III)胺素都需要被未知因子还原后，该酶才能催化下一个循环。

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