产品内容
本酶催化的反应为(1) (2R,3R,5R)-2-(S-Pantetheinyl)-Carbapenam-3-Carboxylate + 2 S-Adenosyl-L-Methionine + Reduced Acceptor = (2R,3R,5S,6R)-6-(Methyl)-2-(S-Pantetheinyl)-Carbapenam-3-Carboxylate + 5'-Deoxyadenosine + L-Methionine + S-Adenosyl-L-Homocysteine + Oxidized Acceptor (Overall Reaction);;(1a) S-Adenosyl-L-Methionine + Cob(I)alamin = S-Adenosyl-L-Homocysteine + Methylcob(III)alamin;;(1b) Methylcob(III)alamin + (2R,3R,5R)-2-(S-Pantetheinyl)-Carbapenam-3-Carboxylate + S-Adenosyl-L-Methionine = Cob(III)alamin + (2R,3R,5S,6R)-6-(Methyl)-2-(S-Pantetheinyl)-Carbapenam-3-Carboxylate + 5'-Deoxyadenosine + L-Methionine;;(1c) Cob(III)alamin + Reduced Acceptor = Cob(I)alamin + Oxidized Acceptor;;(2) (2R,3R,5S,6R)-6-(Methyl)-2-(S-Pantetheinyl)-Carbapenam-3-Carboxylate + 2 S-Adenosyl-L-Methionine + Reduced Acceptor = (2R,3R,5S,6R)-6-(Ethyl)-2-(S-Pantetheinyl)-Carbapenam-3-Carboxylate + 5'-Deoxyadenosine + L-Methionine + S-Adenosyl-L-Homocysteine + Oxidized Acceptor (Overall Reaction);;(2a) S-Adenosyl-L-Methionine + Cob(I)alamin = S-Adenosyl-L-Homocysteine + Methylcob(III)alamin;;(2b) Methylcob(III)alamin + (2R,3R,5S,6R)-6-(Methyl)-2-(S-Pantetheinyl)-Carbapenam-3-Carboxylate + S-Adenosyl-L-Methionine = Cob(III)alamin + (2R,3R,5S,6R)-6-(Ethyl)-2-(S-Pantetheinyl)-Carbapenam-3-Carboxylate + 5'-Deoxyadenosine + L-Methionine;;(2c) Cob(III)alamin + Reduced Acceptor = Cob(I)alamin + Oxidized Acceptor;;系统名称为S-Adenosyl-L-Methionine:(2R,3R,5R)-2-(S-Pantetheinyl)-Carbapenam-3-Carboxylate 6-C-Methyltransferase，一种 radical SAM（AdoMet）酶，在复杂碳青霉烯类抗生素的生物合成过程中催化两次连续甲基化。该酶在 6 位添加甲基，随后第二次甲基化将甲基转化为乙基。该酶结合 [4Fe-4S] 簇，需要钴胺素辅因子和电子供体。体外可使用甲基紫精。
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