本酶催化的反应为L-Threonylcarbamoyladenylate + Adenine37 In TRNA = AMP + N6-L-Threonylcarbamoyladenine37 In TRNA,系统名称为L-Threonylcarbamoyladenylate:adenine37 In TRNA N6-L-Threonylcarbamoyltransferase,该酶参与tRNA中N6-苏氨酰氨基甲酰腺苷37的合成,该修饰存在于反密码子为NNU的tRNA中,即tRNAIle、tRNAThr、tRNAAsn、tRNALys、tRNASer和tRNAArg [3]。