产品内容
本酶催化的反应为(1) 3 Malonyl-CoA + 2 (S)-Methylmalonyl-CoA + Icosanoyl-[(Phenol)carboxyphthiodiolenone Synthase] + 5 NADPH = C32-Carboxyphthiodiolenone-[(Phenol)carboxyphthiodiolenone Synthase] + 5 CoA + 5 NADP+ + 5 CO2 + 2 H2O;;(2) 3 Malonyl-CoA + 2 (S)-Methylmalonyl-CoA + Docosanoyl-[(Phenol)carboxyphthiodiolenone Synthase] + 5 NADPH = C34-Carboxyphthiodiolenone-[(Phenol)carboxyphthiodiolenone Synthase] + 5 CoA + 5 NADP+ + 5 CO2 + 2 H2O;;(3) 3 Malonyl-CoA + 2 (S)-Methylmalonyl-CoA + 19-(4-Hydroxyphenyl)-Nonadecanoyl-[(Phenol)carboxyphthiodiolenone Synthase] + 5 NADPH = C37-(Phenol)carboxyphthiodiolenone-[(Phenol)carboxyphthiodiolenone Synthase] + 5 CoA + 5 NADP+ + 5 CO2 + 2 H2O;;(4) 3 Malonyl-CoA + 2 (S)-Methylmalonyl-CoA + 17-(4-Hydroxyphenyl)heptadecanoyl-[(Phenol)carboxyphthiodiolenone Synthase] + 5 NADPH = C35-(Phenol)carboxyphthiodiolenone-[(Phenol)carboxyphthiodiolenone Synthase] + 5 CoA + 5 NADP+ + 5 CO2 + 2 H2O，系统名称为(Methyl)malonyl-CoA:long-Chain Acyl-[(Phenol)carboxyphthiodiolenone Synthase] (Methyl)malonyltransferase {Carboxyphthiodiolenone-[(Phenol)carboxyphthiodiolenone Synthase]-Forming}，该酶是五个聚酮合酶蛋白的复合体，参与结核二醇和酚结核二醇共同的脂质核心的合成。第一个蛋白 PpsA 可以接受 C18 或 C20 长链脂肪酰基，或 (4-羟基苯基)-C17 或 C19 脂肪酰基。底物必须首先被 EC 6.2.1.59（长链脂肪酸腺苷酰化酶/转移酶 FadD26）腺苷酰化，该酶也将它们装载到 PpsA 上。PpsA 然后用丙二酰-CoA 延伸单元进行延伸。PpsB 蛋白添加下一个丙二酰-CoA 延伸单元。PpsA 和 PpsB 模块中缺乏脱水酶和烯酰还原酶结构域导致形成结核二醇部分中的二醇部分。PpsC 添加第三个丙二酰单元（由于其脱水酶结构域而释放一个水分子），PpsD 添加一个 (R)-甲基丙二酰单元并释放一个水分子，PpsE 添加第二个 (R)-甲基丙二酰单元而不释放水分子。甲基丙二酰单元的掺入导致延伸产物中形成两个分支甲基。该酶不含硫酯酶结构域 [2]，产物的释放需要 tesA 编码的 II 型硫酯酶 [1]。
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