产品内容
本酶催化的反应为4-Methyl-2-Oxopentanoate + Malonyl-[Acp] + A [Lipoyl-Carrier Protein]-N6-[(R)-Lipoyl]-L-Lysine = 5-Methyl-3-Oxohexanoyl-[Acp] + A [Lipoyl-Carrier Protein]-N6-[(R)-Dihydrolipoyl]-L-Lysine + 2 CO2 (Overall Reaction);;(1a) 4-Methyl-2-Oxopentanoate + A [Lipoyl-Carrier Protein]-N6-[(R)-Lipoyl]-L-Lysine = A [Lipoyl-Carrier Protein]-N6-[(3-Methyl-2-Oxobutanoyl)-(R)-Lipoyl]-L-Lysine + CO2;;(1b) A [Lipoyl-Carrier Protein]-N6-[(3-Methyl-2-Oxobutanoyl)-(R)-Lipoyl]-L-Lysine + Malonyl-[Acp] = 5-Methyl-3-Oxohexanoyl-[Acp] + A [Lipoyl-Carrier Protein]-N6-[(R)-Dihydrolipoyl]-L-Lysine + CO2，系统名称为Branched-Chain 2-Oxoacid:malonyl-[Acyl-Carrier Protein] Acyltransferase，该酶从环境 DNA 样品中鉴定并在 Paraburkholderia graminis 中表达，是一种复杂的酶，其目的是通过产生支链 3-氧代酰基-[acp] 分子，将支链 2-氧代酸转向支链 N-酰基氨基酸的生产。NasB 接受支链 2-氧代酸，如 4-甲基-2-氧代戊酸，催化脱羧转移至硫胺素二磷酸辅因子，随后转移至硫辛酰辅因子，最后第二次脱羧转移至丙二酰-[acp] 受体。产物（如 5-甲基-3-氧代己酰基-[acp]）可以被脂肪酸合酶系统延伸，形成支链长链脂肪酰基-[acp] 分子，这些分子作为 EC 2.3.1.327（长链酰基-[acp]:L-苯丙氨酸 N-酰基转移酶，NasA）的底物。在反应过程中，硫辛酰辅因子被还原为二氢硫辛酰，必须由一种未知酶将其氧化回硫辛酰。
![天净沙支链2-氧代酸:丙二酰-[酰基载体蛋白]酰基转移酶活性测定试剂盒产品图片](https://www.tjs.bio/content/xtheme/tianjingsha/images/noimage.jpg)
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