本酶催化的反应为Endonucleolytic Cleavage Of RNA, Removing Extra 3' Nucleotides From TRNA Precursor, Generating 3' Termini Of TRNAs. A 3'-Hydroxy Group Is Left At The TRNA Terminus And A 5'-Phosphoryl Group Is Left At The Trailer Molecule，无辅因子需求。在詹氏甲烷球菌（Methanococcus jannaschii）中发现了与拟南芥（Arabidopsis thaliana）中发现的酶同源的酶。
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