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本酶催化的反应为Release Of An N-Terminal Amino Acid, Xaa!Yaa-, In Which Xaa Is Preferably Leu, But May Be Other Amino Acids Including Pro Although Not Arg Or Lys, And Yaa May Be Pro. Amino Acid Amides And Methyl Esters Are Also Readily Hydrolysed, But Rates On Arylamides Are Exceedingly Low，一种从猪肾和牛晶状体中分离的锌酶；受重金属离子激活。肽酶家族M17的典型代表。
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