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本酶催化的反应为Hydrolyses Glutaminyl Bonds, And Activity Is Further Restricted By Preferences For The Amino Acids In P6 - P1' That Vary With The Species Of Potyvirus, E.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln!(Ser Or Gly) For The Enzyme From Tobacco Etch Virus. The Natural Substrate Is The Viral Polyprotein, But Other Proteins And Oligopeptides Containing The Appropriate Consensus Sequence Are Also Cleaved.马铃薯Y病毒在植物中引起疾病，宿主细胞核中出现包涵体；包涵体的a蛋白就是内肽酶。该酶在编码重组融合蛋白人工表达的载体中具有实际用途，因为它可以赋予融合蛋白自溶切割的能力。也有报道称表达该酶的转基因植物对病毒感染具有抗性。肽酶家族C4的典型代表。
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