本酶催化的反应为All Bonds Known To Be Hydrolysed By This Endopeptidase Have Arginine In P1 And An Acidic Residue In P4. P6 Is Often Occupied By An Acidic Residue Or By An Hydroxy-Amino-Acid Residue, The Phosphorylation Of Which Enhances Cleavage，在芽殖酵母和人类细胞中，分离酶（separase）切割黏连蛋白亚基Scc1是有丝分裂中姐妹染色单体分离所必需的。芽殖酵母分离酶也已知切割减数分裂黏连蛋白复合物的Rec8亚基和动粒蛋白Slk19。肽酶家族C50的典型代表。
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