产品内容
本酶催化的反应为(1) [LarE]-L-Cysteine + Pyridin-1-Ium-3,5-Dicarboxylate Mononucleotide + ATP = [LarE]-Dehydroalanine + Pyridin-1-Ium-3-Carboxylate-5-Thiocarboxylate Mononucleotide + AMP + Diphosphate (Overall Reaction);;(1a) ATP + Pyridin-1-Ium-3,5-Dicarboxylate Mononucleotide = Diphosphate + 5-Carboxy-1-(5-O-Phospho-beta-D-Ribofuranosyl)pyridin-1-Ium-3-Carbonyl Adenylate;;(1b) 5-Carboxy-1-(5-O-Phospho-beta-D-Ribofuranosyl)pyridin-1-Ium-3-Carbonyl Adenylate + [LarE]-L-Cysteine = AMP + [LarE]-S-[5-Carboxy-1-(5-O-Phosphono-beta-D-Ribofuranosyl)pyridin-1-Ium-3-Carbonyl]-L-Cysteine;;(1c) [LarE]-S-[5-Carboxy-1-(5-O-Phosphono-beta-D-Ribofuranosyl)pyridin-1-Ium-3-Carbonyl]-L-Cysteine = [LarE]-Dehydroalanine + Pyridin-1-Ium-3-Carboxylate-5-Thiocarboxylate Mononucleotide;;(2) [LarE]-L-Cysteine + Pyridin-1-Ium-3-Carboxylate-5-Thiocarboxylate Mononucleotide + ATP = [LarE]-Dehydroalanine + Pyridin-1-Ium-3,5-Bisthiocarboxylate Mononucleotide + AMP + Diphosphate (Overall Reaction);;(2a) ATP + Pyridin-1-Ium-3-Carboxylate-5-Thiocarboxylate Mononucleotide = Diphosphate + 1-(5-O-Phospho-beta-D-Ribofuranosyl)-5-(Sulfanylcarbonyl)pyridin-1-Ium-3-Carbonyl Adenylate;;(2b) 1-(5-O-Phospho-beta-D-Ribofuranosyl)-5-(Sulfanylcarbonyl)pyridin-1-Ium-3-Carbonyl Adenylate + [LarE]-L-Cysteine = AMP + [LarE]-S-[1-(5-O-Phosphono-beta-D-Ribofuranosyl)-5-(Sulfanylcarbonyl)pyridin-1-Ium-3-Carbonyl]-L-Cysteine;;(2c) [LarE]-S-[1-(5-O-Phosphono-beta-D-Ribofuranosyl)-5-(Sulfanylcarbonyl)pyridin-1-Ium-3-Carbonyl]-L-Cysteine = [LarE]-Dehydroalanine + Pyridin-1-Ium-3,5-Bisthiocarboxylate Mononucleotide，系统名称为[LarE]-S-[1-(5-O-Phosphono-beta-D-Ribofuranosyl)-5-(Sulfanylcarbonyl)pyridin-1-Ium-3-Carbonyl]-L-Cysteine Pyridin-1-Ium-3,5-Dicarbothioate-Mononucleotide-Lyase (ATP-Consuming)，该酶存在于植物乳杆菌（Lactobacillus plantarum）中，参与镍钳口辅因子的生物合成。过程开始于一个酶分子腺苷酰化吡啶鎓-3,5-二羧酸单核苷酸（P2CMN），并将腺苷酰化产物共价结合到内在的半胱氨酸残基上。接着，该酶切断碳-硫键，释放吡啶鎓-3-羧酸-5-硫代羧酸单核苷酸（PCTMN），并在蛋白上留下 2-氨基丙烯-2-酸（脱氢丙氨酸）残基。由于半胱氨酸残基在体内不会再生，该酶在此过程中失活。随后第二个酶分子对 PCTMN 重复该过程，将其腺苷酰化并共价结合到同一半胱氨酸残基上，随后释放吡啶鎓-3,5-二硫代羧酸单核苷酸（P2TMN），第二个酶分子也随之失活。参见 EC 4.4.1.45（外在半胱氨酸依赖吡啶鎓-3,5-二硫代羧酸单核苷酸合酶）。
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