产品内容
本酶催化的反应为(1) A [4Fe-5S] Iron-Sulfur Cluster Linked By 3 L-Cysteine Residues + Pyridin-1-Ium-3,5-Dicarboxylate Mononucleotide + ATP + Reduced Acceptor = A [4Fe-4S] Iron-Sulfur Cluster Linked By 3 L-Cysteine Residues + Pyridin-1-Ium-3-Carboxylate-5-Thiocarboxylate Mononucleotide + AMP + Diphosphate + Acceptor (Overall Reaction);;(1a) ATP + Pyridin-1-Ium-3,5-Dicarboxylate Mononucleotide = Diphosphate + 5-Carboxy-1-(5-O-Phospho-beta-D-Ribofuranosyl)pyridin-1-Ium-3-Carbonyl Adenylate;;(1b) A [4Fe-5S] Iron-Sulfur Cluster Linked By 3 L-Cysteine Residues + 5-Carboxy-1-(5-O-Phospho-beta-D-Ribofuranosyl)pyridin-1-Ium-3-Carbonyl Adenylate + Reduced Acceptor = A [4Fe-4S] Iron-Sulfur Cluster Linked By 3 L-Cysteine Residues + Pyridin-1-Ium-3-Carboxylate-5-Thiocarboxylate Mononucleotide + AMP + Acceptor;;(2) A [4Fe-5S] Iron-Sulfur Cluster Linked By 3 L-Cysteine Residues + Pyridin-1-Ium-3-Carboxylate-5-Thiocarboxylate Mononucleotide + ATP + Reduced Acceptor = A [4Fe-4S] Iron-Sulfur Cluster Linked By 3 L-Cysteine Residues + Pyridin-1-Ium-3,5-Bisthiocarboxylate Mononucleotide + AMP + Diphosphate + Acceptor (Overall Reaction);;(2a) ATP + Pyridin-1-Ium-3-Carboxylate-5-Thiocarboxylate Mononucleotide = Diphosphate + 1-(5-O-Phospho-beta-D-Ribofuranosyl)-5-(Sulfanylcarbonyl)pyridin-1-Ium-3-Carbonyl Adenylate;;(2b) A [4Fe-5S] Iron-Sulfur Cluster Linked By 3 L-Cysteine Residues + 1-(5-O-Phospho-beta-D-Ribofuranosyl)-5-(Sulfanylcarbonyl)pyridin-1-Ium-3-Carbonyl Adenylate + Reduced Acceptor = A [4Fe-4S] Iron-Sulfur Cluster Linked By 3 L-Cysteine Residues + Pyridin-1-Ium-3,5-Bisthiocarboxylate Mononucleotide + AMP + Acceptor，系统名称为Pyridin-1-Ium-3,5-Bisthiocarboxylate Mononucleotide Sulfo-Lyase，这种酶存在于细菌 Thermotoga maritima 中，在镍钳辅因子的生物合成过程中催化两个复杂反应。该过程始于吡啶-1-鎓-3,5-二甲酸单核苷酸（P2CMN）的腺苷酰化，P2CMN 共价结合至固有的半胱氨酸残基。接下来，[4Fe-4S] 铁硫簇通过 EC 2.8.1.7（半胱氨酸脱硫酶）的作用从游离 L-半胱氨酸接受硫烷硫，形成暂时的 [4Fe-5S] 簇。然后硫原子攻击活化的底物，导致形成吡啶-1-鎓-3-羧酸-5-硫代羧酸单核苷酸（PCTMN）。该过程重复两次，最终产物为吡啶-1-鎓-3,5-双硫代羧酸单核苷酸（P2TMN）。参见 EC 4.4.1.37，固有半胱氨酸依赖性吡啶鎓-3,5-双硫代羧酸单核苷酸合酶。
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