产品内容
本酶催化的反应为(1) An [RNA] Containing Cytidine + H2O = An [RNA]-3'-Cytidine-3'-Phosphate + A 5'-Hydroxy-Ribonucleotide-3'-[RNA] (Overall Reaction);;(1a) An [RNA] Containing Cytidine = An [RNA]-3'-Cytidine-2',3'-Cyclophosphate + A 5'-Hydroxy-Ribonucleotide-3'-[RNA];;(1b) An [RNA]-3'-Cytidine-2',3'-Cyclophosphate + H2O = An [RNA]-3'-Cytidine-3'-Phosphate;;(2) An [RNA] Containing Uridine + H2O = An [RNA]-3'-Uridine-3'-Phosphate + A 5'-Hydroxy-Ribonucleotide-3'-[RNA];;(2a) An [RNA] Containing Uridine = An [RNA]-3'-Uridine-2',3'-Cyclophosphate + A 5'-Hydroxy-Ribonucleotide-3'-[RNA];;(2b) An [RNA]-3'-Uridine-2',3'-Cyclophosphate + H2O = An [RNA]-3'-Uridine-3'-Phosphate，系统名称为RNA Lyase ([RNA]-3'-Cytidine/uridine-3'-Phosphate And 5'-Hydroxy-Ribonucleotide-3'-[RNA] Producing)，特异性切割 RNA 中嘧啶（尿嘧啶或胞嘧啶）磷酸键的 3'-侧。反应分两步进行，第一步完成时 2',3'-环状磷酸二酯中间体从酶上释放。这些环状化合物的水解通过第一步的逆反应发生，速度慢得多——在该逆反应中，水的 -OH 基取代第一步中核糖的 2'-OH 基，并且直到几乎所有可被切割的 3',5'-磷酸二酯键都被环化后才发生。该酶可以作为内切或外切核糖核酸酶发挥作用。
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