产品内容
本酶催化的反应为(1) [RNA] Containing Adenosine + H2O = An [RNA Fragment]-3'-Adenosine-3'-Phosphate + A 5'-Hydroxy-Ribonucleotide-3'-[RNA Fragment] (Overall Reaction);;(1a) [RNA] Containing Adenosine = An [RNA Fragment]-3'-Adenosine-2',3'-Cyclophosphate + A 5'-Hydroxy-Ribonucleotide-3'-[RNA Fragment];;(1b) An [RNA Fragment]-3'-Adenosine-2',3'-Cyclophosphate + H2O = An [RNA Fragment]-3'-Adenosine -3'-Phosphate;;(2) [RNA] Containing Guanosine + H2O = An [RNA Fragment]-3'-Guanosine-3'-Phosphate + A 5'-Hydroxy-Ribonucleotide-3'-[RNA Fragment] (Overall Reaction);;(2a) [RNA] Containing Guanosine = An [RNA Fragment]-3'-Guanosine-2',3'-Cyclophosphate + A 5'-Hydroxy-Ribonucleotide-3'-[RNA Fragment];;(2b) An [RNA Fragment]-3'-Guanosine-2',3'-Cyclophosphate + H2O = An [RNA Fragment]-3'- Guanosine-3'-Phosphate，系统名称为[RNA]-Purine 5'-Hydroxy-Ribonucleotide-3'-[RNA Fragment]-Lyase (Cyclicizing; [RNA Fragment]-3'-Purine-Nucleoside -2',3'-Cyclophosphate-Forming And Hydrolysing)，由真菌球形黑粉菌（Ustilago sphaerogena）分泌的该酶在嘌呤的 3'-磷酸基团处切割，催化两阶段的内切核水解切割。第一步反应产生 5'-羟基-磷酸寡核苷酸和以 Ap 或 Gp（带 2',3'-环状磷酸二酯）结尾的 3'-磷酸寡核苷酸，它们从酶上释放。然后，只有当所有可被切割的 3',5'-磷酸二酯键都被环化后，酶才在第二步反应中水解这些环状化合物。第二步是第一步的逆反应，使用水的羟基代替核糖的 5'-羟基。整个过程是磷-氧裂合酶作用后接着水解，形成 3'-核苷酸。
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