Name: 天净沙DNA连接酶(ATP、ADP或GTP)活性测定试剂盒
Brand: 天净沙
SKU: 7-EC6.5.1.7
Availability: InStock

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天净沙DNA连接酶(ATP、ADP或GTP)活性测定试剂盒

英文名：Skygen DNA Ligase (ATP, ADP Or GTP) Assay Kit

别名：Poly(deoxyribonucleotide):poly(deoxyribonucleotide) Ligase (ATP, ADP Or GTP)

产品编号：7-EC6.5.1.7

保存温度：-20℃

运输温度：Cold

货号

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货期

目录价

7-EC6.5.1.7

100 T

3-4 Weeks

本酶催化的反应为(1) ATP + (Deoxyribonucleotide)n-3'-Hydroxyl + 5'-Phospho-(Deoxyribonucleotide)m = (Deoxyribonucleotide)n+m + AMP + Diphosphate (Overall Reaction);;(1a) ATP + [DNA Ligase]-L-Lysine = 5'-Adenosyl [DNA Ligase]-Nepsilon-Phosphono-L-Lysine + Diphosphate;;(1b) 5'-Adenosyl [DNA Ligase]-Nepsilon-Phosphono-L-Lysine + 5'-Phospho-(Deoxyribonucleotide)m = 5'-(5'-Diphosphoadenosine)-(Deoxyribonucleotide)m + [DNA Ligase]-L-Lysine;;(1c) (Deoxyribonucleotide)n-3'-Hydroxyl + 5'-(5'-Diphosphoadenosine)-(Deoxyribonucleotide)m = (Deoxyribonucleotide)n+m + AMP;;(2) ADP + (Deoxyribonucleotide)n-3'-Hydroxyl + 5'-Phospho-(Deoxyribonucleotide)m = (Deoxyribonucleotide)n+m + AMP + Phosphate (Overall Reaction);;(2a) ADP + [DNA Ligase]-L-Lysine = 5'-Adenosyl [DNA Ligase]-Nepsilon-Phosphono-L-Lysine + Phosphate;;(2b) 5'-Adenosyl [DNA Ligase]-Nepsilon-Phosphono-L-Lysine + 5'-Phospho-(Deoxyribonucleotide)m = 5'-(5'-Diphosphoadenosine)-(Deoxyribonucleotide)m + [DNA Ligase]-L-Lysine;;(2c) (Deoxyribonucleotide)n-3'-Hydroxyl + 5'-(5'-Diphosphoadenosine)-(Deoxyribonucleotide)m = (Deoxyribonucleotide)n+m + AMP;;(3) GTP + (Deoxyribonucleotide)n-3'-Hydroxyl + 5'-Phospho-(Deoxyribonucleotide)m = (Deoxyribonucleotide)n+m + GMP + Diphosphate (Overall Reaction);;(3a) GTP + [DNA Ligase]-L-Lysine = 5'-Guanosyl [DNA Ligase]-Nepsilon-Phosphono-L-Lysine + Diphosphate;;(3b) 5'-Guanosyl [DNA Ligase]-Nepsilon-Phosphono-L-Lysine + 5'-Phospho-(Deoxyribonucleotide)m = 5'-(5'-Diphosphoguanosine)-(Deoxyribonucleotide)m + [DNA Ligase]-L-Lysine;;(3c) (Deoxyribonucleotide)n-3'-Hydroxyl + 5'-(5'-Diphosphoguanosine)-(Deoxyribonucleotide)m = (Deoxyribonucleotide)n+m + GMP，系统名称为Poly(deoxyribonucleotide)-3'-Hydroxyl:5'-Phospho-Poly(deoxyribonucleotide) Ligase (ATP, ADP Or GTP)，来自古菌 Hyperthermus butylicus 和 Sulfophobococcus zilligii 的这些酶对ATP、ADP或GTP有活性，对NAD+无活性。该酶催化连接具有3'-羟基和5'-磷酸末端的DNA链，形成磷酸二酯键并封闭双链DNA中某些类型的单链断裂。催化按三步机制进行：首先ATP、ADP或GTP激活酶，在腺苷酸/鸟苷酸与一个赖氨酸残基之间形成磷酰胺键；随后核苷酸转移到底物的5'-磷酸末端，形成加帽结构5'-(5'-二磷酸腺苷/鸟苷)-[DNA]；最后该酶催化3'-OH末端对加帽末端的亲核进攻，形成磷酸二酯键并释放核苷酸。与 EC 6.5.1.1（DNA连接酶（ATP））和不能利用GTP的 EC 6.5.1.6（DNA连接酶（ATP或NAD+））不同。

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